HB-10 Workshops

 

Tuesday, September 5th, 2017 Theme:           Workshops Chair:              Michalis Aivaliotis Lecture Hall:  Alkiviades Payatakis Morning workshop   09:00-13:00 Proteomics Workshop – Hands-on Proteomics Bioinformatics: From raw MS-data to knowledge Chair: Michalis Aivaliotis 09:00-09:15 Michalis Aivaliotis Introduction to proteomics Brief history of MS-based proteomics State-of-the-art proteomics 09:15-10:30 M. Aivaliotis, N. Kountourakis, K. Psatha, C. Seitanidou Bioinformatics I – MS-Raw data processing Proteome Identification and quantitation using commercial available and free bioinformatic platforms Quality control and filtering of proteomics data 10:30-13:00 M. Aivaliotis, N. Kountourakis, K. Psatha, C. Seitanidou Bioinformatics II – Meta-data analysis and visualization Comparative proteomics analysis Functional proteome annotation GO terms and pathways enrichment analysis Protein-protein interaction networks Visualization of proteomics data 13:00-14:00 Lunch Break Evening workshop 14:00-18:00 Advanced RNA-Seq and ChIP-Seq data analysis – Hands-on Chair: Pantelis Topalis 14:00-14:15 Pantelis Topalis Introduction to RNA-Seq and ChIP-Seq Experimental Design and Data analysis Quality Control 14:15-16:00 P. Topalis, E. Dialynas, G. Papagiannakis RNA-Seq Data Analysis De novo transcriptome assembly Differential Gene Expression Everything you always wanted to know about batch effects but you were afraid to ask 16:00-18:00 P. Topalis, E. Dialynas ChIP-Seq Data Analysis Peak finding (Transcription Factors, Histone modifications, Super Enhancers) Irreproducible Discovery Rate (IDR) pipeline Peak annotation Important Notes: Number of maximum participants 35. A digital textbook and references will be provided to all participants in advance. All the participants need to have their own laptops. No computers will be provided by the organizers.